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Bone marrow (BM) cells cultured for 14 days in the presence of 10% fetal calf serum (FCS) showed cells with chromosome numbers between 40 and 60 with a near diploid karyotype. Cells cultured in the presence of 3% FCS showed variable chromosome numbers. Three karyotypes were found, 48.10, 48.14, and 58.00. Cells with a chromosome number of 58 showed the presence of a 42-line marker chromosome, which indicated some infidelity of mitosis. The application of various techniques showed that these results are reliable. Thus, a simple procedure for karyotyping cells in culture is presented.Prevalence and risk factors of glucose intolerance in active adolescents with type 2 diabetes mellitus: a national population


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