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Global gene expression profile of the human submandibular gland during normal progression of age.
Understanding the changes in gene expression profile with age is an important aspect of aging biology. To understand the overall gene expression pattern associated with normal aging of the human submandibular gland, we performed a cDNA microarray analysis using a mixture of 14 different human tissue samples, including the human submandibular gland, and using a cDNA microarray (G3442A, Applied Biosystems) that includes a total of 4,515 genes. We observed a total of 56 transcripts whose expression was changed with age. Of these, 40 transcripts were up-regulated and 16 were down-regulated. Moreover, we identified some candidate molecules that could be useful for understanding the aging process. The most up-regulated gene with aging was the human homologue of the Drosophila achaete-scute complex. Many of the genes involved in defense response were also up-regulated with age, suggesting that this tissue undergoes changes in the defense response with aging. Many of the genes that were down-regulated with aging encoded proteins that function as negative regulators of cell division. We believe that this profile of genes will be useful for exploring the mechanisms of aging in this tissue.
76 B.R. 832 (1987)
In the Matter of DALLAS COUNTY FRONTAGE MECHANICAL CO., INC., Debtor.
Bankruptcy No. 87-40189.
United States Bankruptcy Court, N.D. Indiana, Fort Wayne Division.
August 14, 1987.
Jeffrey M. Miller, Fort Wayne, Ind